Development of a duplex real-time recombinase aided amplification assay for the simultaneous and rapid detection of PCV3 and PCV4 | Virology Journal
Standard plasmid for duplex real-time RAA The complete genome of PCV3 (2, 000 bp) and PCV4 (1, 770 bp) were synthesized according to the PCV3 and PCV4 genomic sequence (GenBank accession no. KT869077 and MT311852) and cloned into the pUC57 vector by Sangon Biotech Co., Ltd. (Sangon Biotech Co., Ltd., Shanghai, China) to construct standard plasmids for … Read more