Hilger, D., Masureel, M. & Kobilka, B. K. Structure and dynamics of GPCR signaling complexes. Nat. Struct. Mol. Biol. 25, 4–12 (2018). Article CAS PubMed PubMed Central Google Scholar Herrera, L. P. T. et al. GPCRdb in 2025: adding odorant receptors, data mapper, structure similarity search and models of physiological ligand complexes. Nucleic Acids Res. … Read more
Biosensor construction Gibson assembly All cloning, except for site-directed mutagenesis, was conducted using Gibson assembly (NEBuilder HiFi DNA Assembly Master Mix, New England Biolabs)54. All primers were synthesized by BioTeZ. A list of all of the primer sequences is provided in Supplementary Table 5. Primers 1 and 3 were used to cut within the pcDNA3.0 … Read more
Credit: igoriss / iStock / Getty Images Plus A new study from researchers at the University of Pittsburgh and Yale University shows how common bacteria found in the human gut can influence how medications that target G protein-coupled receptors (GPCRs) are metabolized, rendering them less effective. The study, published in Nature Chemistry, could have implications … Read more