Animals and cell lines
Animal work
Mouse (Mus musculus) strains C57BL/6J (strain no. 000664) and Cdx2:CreERT2;APCfl/fl;KrasWT (strain no. 035169)50 were obtained from The Jackson Laboratory. Mice were housed at room temperature and ambient humidity in individually ventilated cages at a maximum density of five mice per cage with ad libitum access to food and water in a specific-pathogen-free facility accredited by the Association and Accreditation of Laboratory Animal Committee. Cages contained Anderson’s Bed-o’Cob bedding (The Anderson Inc.), one nestlet (Ancare, 2 × 2-inch2 compressed cotton squares) and a red mouse hut (Bioserv). The colony room was kept on a 12 h–12 h light–dark cycle. All animal handling and experiments were conducted in accordance with procedures approved by the Institutional…